If you plan to update/remove mp3 file tags, without box checking there will no any tags updating/removing. If you run copying/renaming operations, both ID3v1 and ID3v2 tags will be updated without any box checking.

Other Topics
What purpose of "File#" copy method option?
Who designed id3 tags for mp3 files?
What difference between track-at-once and disc-at-once within Audio CD Burning?
Where can I view or download the source code of Mp3 ID3v1v2 Tag Edit & Sort Tool?